Fig. 7
From: High plasticity of axonal pathology in Alzheimer’s disease mouse models
Correlative two-photon microscopy and TEM of an Aβ plaque. (a), Maximum projection of images taken from a GFP-expressing AxD near an Aβ plaque stained with Methoxy-X04 (blue) on day 162 (the same AxD (dys 7) as in Fig. 1a-c – red arrow). (b), Ex vivo single plane of the AxD (note that some NIRB marks were made around the region of interest to locate it in subsequent processing steps; marks have been labeled with asterisks). (c), Low-magnification electron micrograph showing the Aβ plaque (the central core formed by fibrillar Aβ peptide has been pseudocolored in blue). The GFP-expressing AxD (dys 7) that was imaged in vivo is surrounded by the laser marks (pseudocolored in orange and labeled with asterisks). Rectangles delimit the regions shown at a higher magnification in panels d, e. (d), Image showing a microglial cell with numerous phagocytic inclusions (arrows) in close apposition to an AxD, suggesting that this cell is participating in phagocytosis of the AxD. Microglial cell was identified based on its ultrastructural characteristics. (e), Higher magnification of dys 7. Note that autophagic vacuoles take up a large area of the AxD contents, and also that the AxD is almost devoid of any normal-looking organelles. A normal-looking asymmetric synapse can be observed in close apposition to the AxD (arrowhead). Scale bar (in e): 23.1 μm in a, b; 3 μm in c; 0.61 μm in d; 0.52 μm in e