Fig. 2

HSPB1 mutants do not show differences in general protein translation. a The polysome gradient analysis was performed on a 5-50% sucrose gradient with protein lysates originating from patient- and control-derived lymphoblastoid cell lines. b-d The isolated fractions originating from the polysome gradient analysis were assessed by western blotting. Rps6 was used as a ribosomal marker. The HSPB1 band visible in fraction 1 of the control individual (b) is due to technical variation