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Table 2 Antibodies

From: Threonine175, a novel pathological phosphorylation site on tau protein linked to multiple tauopathies

Antibody Clone Titre Antigen retrieval Epitope Company
Tau pThr175 Rabbit, polyclonal 1:1000 1 pThr175 21st Century
Tau pThr217 Rabbit, polyclonal 1:1000 1 pThr217 21st Century
Tau pSer208, 210 Rabbit, polyclonal 1:1000 1 pSer208, 210 21st Century
PHF (AT8) Mouse, monoclonal 2.5 ug/ml No pSer202 Thermo Fischer
Tau pThr231 Rabbit, polyclonal 1:1000 2 pThr231 Thermo Fischer
T22 Rabbit, polyclonal 1:500 2 Tau oligomer EMD Millipore
Alexa Fluor 488 Goat anti-rabbit 1:200 2 Secondary Life Technologies
  1. 1) Boil in 10 mM sodium citrate, 0.05% Tween 20 pH 6.0 for 2 min
  2. 2) Pressure cooker (2100 Retriever; Aptum Biologics, UK) 10 mM sodium citrate, 0.05% Tween 20 pH 6.0 for 15 min