Fig. 5

Exogenous Tau fibrils are found within TNTs. CAD neuronal cells (a) or primary neurons (b) were plated in Lab-Tek chamber slides and infected with LVs encoding mCherry-Actin (red). At 48 h post-infection, cells were incubated for six hours with extracellular ATTO 488-hTau1N4R fibrils/liposomes preparation (green). Cells were processed for immunostaining analysis using an anti-acetylated tubulin visualized with an Alexa 647-labeled secondary antibody (white). Nuclei were labeled with DAPI (blue). TNTs containing Tau are shown in enlargements (white arrows). For acquisition, a focal plane was collected for specimen. Images were acquired with an inverted laser-scanning confocal microscope using a 40× oil-immersion lens (NA 1.3) and processed using the programs ZEN and ImageJ. Scale bars: 10 μm