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Table 2 List of antibodies used in this study

From: Tau pathology in aged cynomolgus monkeys is progressive supranuclear palsy/corticobasal degeneration- but not Alzheimer disease-like -Ultrastructural mapping of tau by EDX-

Antibody (clone)

Epitope

Species

Dilution

Pretreatment

Supplier

PHF-tau (AT8)

pS202/pT205(/pS208) [17, 18]

mo

1:10,000

none

Thermo Fisher, Waltham, MA

RD3 (8E6/C11)

KHQPGGGKVQIVYKPV [11]

mo

1:3000

KMn, Ox, FA, AC [12]

Upstate, Lake Placid, NY

RD4 (1E1/A6)

VQIINKKLDLSNVQSKC [11]

mo

1:1000

KMn, Ox, FA, AC [12]

Upstate, Lake Placid, NY

Aβ42

 

rb

1:20,000

FA

IBL, Fujioka, Japan

  1. Abbreviations: mo mouse, KMn 0.25 % potassium permanganate for 15 min, Ox 2%oxalic acid for 3 min, FA 99%formic acid for 30 min, AC autoclaving in 0.01 M citrate buffer at 121 °C for 30 min, rb rabbit