Fig. 5
From: pGluAβ increases accumulation of Aβ in vivo and exacerbates its toxicity
pGluAβ increases accumulation of Aβ in vivo, and exacerbates toxicity (a). Flies expressing Aβ1-42 had significantly more soluble Aβ, P <0.05 but less insoluble Aβ, P <0.0001 in comparison to flies expressing AβpE3-42. Flies co-expressing Aβ1-42 and AβpE3-42 had significantly more insoluble Aβ, P <0.01 but less soluble Aβ P <0.05 Aβ in comparison to flies co-expressing Aβ1-42 and Aβ1-42. Data are presented as means ± SEM and were analysed by Student’s t-test. b There was a significant increase in Aβ1-42 accumulation in flies co-expressing Aβ1-42;AβpE3-42 in comparison to flies expressing either a single copy of Aβ1-42 (expressing one single copy of Aβ1-42 does not accumulate enough protein levels for quantification by western blot analyses), or flies expressing 2 copies of Aβ1-42, P < 0.05. Data are presented as means ± SEM and were analysed by Student’s t test. GMR-GAL4/+;UAS-Aβ1-42/+, GMR-GAL4/+;UAS-AβQ3-42/+, GMR-GAL4/UAS-Aβ1-42;UAS-Aβ1-42/+ and GMR-GAL4/+;UAS-Aβ1-42/UAS-AβQ3-42 flies were used for (a) and (b). c. Co-expression of Aβ1-42 and AβpE3-42 led to a disorganized eye phenotype that was absent in flies co-expressing Aβ1-42 and Aβ1-42, and worse than in AβpE3-42 expressing flies. From left to right, GMR-GAL4/+;UAS-AβQ3-42/+, GMR-GAL4/UAS-Aβ1-42;UAS-Aβ1-42/+ and GMR-GAL4/+;UAS-Aβ1-42/UAS-AβQ3-42 flies. Flies were grown at 25 °C