Fig. 2
From: pGluAβ increases accumulation of Aβ in vivo and exacerbates its toxicity
Expression of AβpE3-42 causes locomotor dysfunction, shortened lifespan, eye disruption, and JNK activation in Drosophila. a Climbing ability of elavGS/UAS-AβQ3-42 flies on + and – RU486 SY medium were assessed at the indicated time-points. Data are presented as the average performance index (PI) ± SEM and were compared using 2-way ANOVA (number of independent tests (n) = 3 *P < 0.001 (b) Expression of AβpE3-42 in adult neurons shortens lifespan. Survival curves are depicted and data were compared using the log-rank test, *P < 0.001 comparing elavGS/UAS-AβQ3-42 + RU flies to -RU flies. c Expression of AβpE3-42 causes a neurodegenerative eye phenotype. First 2 images from left to right are light microscopy images and latter 2 images are nail varnish imprints of eyes (magnification is 25×, and 40× objectives for close up images). From left to right, GMR-GAL4/UAS-EGFP, GMR-GAL4/+;UAS-AβQ3-42/+, GMR-GAL4/+, GMR-GAL4/+;UAS-AβQ3-42/+. Note compressed and fused ommatidia in AβQ3-42 expressing flies in comparison to control flies (GMR-GAL4/UAS-EGFP or GMR-GAL4/+). Flies were grown at 28 °C. d Expression of AβpE3-42 increases the levels of phosphorylated JNK. Data are presented as means ± SEM and were analysed by Student t test, *P < 0.05 comparing GMR-GAL4/+;UAS-AβQ3-42/+ flies to control GMR-GAL4/UAS-LACZ flies