Fig. 2
Tor1a+/- and wt mice do not show structural differences in the peripheral nervous system, spinal cord and brain. a Representative examples of a nerve conduction study in wt and Tor1a+/- control (left) and crush injured (right) mouse sciatic nerves. Magnification of proximal compound action potential in wt and Tor1a+/- crush injured mice illustrates abundant polyphasic late potentials indicating slowly conducting nerve fibre populations. b, c Functional analysis of sciatic nerves by nerve conduction study at the operated ipsilateral (light grey) and the unoperated contralateral (dark grey) side in Tor1a+/- and wt mice with sciatic nerve crush or sham injury. Parameters of interest (mean ± SD) are (b) compound muscle action potential (CMAP) and (c) the nerve conduction velocity (NCV). d, e Diagrams showing optical density of immunohistochemical staining against (d) myelin protein zero (MPZ) and (e) neurofilament (NF) (mean ± SEM). f Diagram showing number of F4/80+ macrophages/mm2 (mean ± SEM) in sciatic nerves of naïve wt, naïve Tor1a+/-, crush injured wt and Tor1a+/- mice. n = number of mice are depicted below the diagrams. Statistical analysis was performed by using the parametric one-way ANOVA with posthoc Tukey test