Table 2 Antibodies used

Primary

Anti-GFAP

Santa Cruz Biotechnolgy, Santa Cruz, CA, USA; #sc-33673

1:500

1:50

Anti-EAAT1 or GLAST

AbCam, Cambridge, UK; #ab416

1:1500

1:250

Anti-glutamine synthetase

AbCam; #ab73593

1:2000

1:333

Anti-VGluT2

Thermo Fisher Scientific, Rockford, IL; USA; #PA5-25653

1:1000

1:50

Anti-GAD65

AbCam; #ab26113

1:2000

1:200

Anti-MBP

Sigma-Aldrich Inc., St. Louis, MO, USA; #M3821

1:500

1:100

Anti-β-actin

AbCam; #ab6276

1:1000

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Secondary

Horseradish peroxidase-conjugated goat anti-rabbit IgG

Thermo Fisher Scientific; #32460

1:200

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Horseradish peroxidase-conjugated goat anti-mouse IgG

Thermo Fisher Scientific; #32430

1:650

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Horseradish peroxidase-conjugated goat anti-mouse IgG2a

Santa Cruz Biotechnolgy; #sc-2061

1:3000

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Biotinylated goat anti-rabbit IgG

Vector Laboratories, Burlingame, CA; #PK-6101

-----

1:200

Biotinyted goat anti-mouse IgG

Vector Laboratories; #PK-6102

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1:200