Skip to main content

Table 2 Summary of mouse tissue used for immunohistochemical analysis of novel antibodies

From: Novel antibodies to phosphorylated α-synuclein serine 129 and NFL serine 473 demonstrate the close molecular homology of these epitopes

Mouse line

Treatment

Fixative

References

M83+/−

Intramuscular injection of αS fibrils (motor impaired/terminal)

150 mM NaCl/ 70 % ethanol

[28]

M83+/−

Intramuscular injection of αS fibrils (motor impaired/terminal)

Formalin

[28]

M83+/−

Cerebral injection of αS fibrils (3 months post-injection)

150 mM NaCl/ 70 % ethanol

[26]

M83+/+

Naïve (7 month old; no phenotype)

150 mM NaCl/ 70 % ethanol

 

M83+/+

Naïve (10–12 month old; motor impaired/terminal)

150 mM NaCl/ 70 % ethanol

 

M20+/−

Cerebral injection of αS fibrils (4 months post-injection)

150 mM NaCl/ 70 % ethanol

[27]

M20+/−

Cerebral injection of αS fibrils (4 months post-injection)

Formalin

[27]

WT

Naïve

150 mM NaCl/ 70 % ethanol