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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Isoaspartic acid is present at specific sites in myelin basic protein from multiple sclerosis patients: could this represent a trigger for disease onset?

Fig. 3

Racemization of Asp34 in MBP from controls () and multiple sclerosis (MS) patients suffering from SPMS () PPMS () and RRMS (). Conversion of L-Asp34 was measured using the tryptic peptide HRDTGILDSIGR to (a) HR(D-isoAsp)TGILDSIGR and (b) HR(L-isoAsp)TGILDSIGR. Elevated racemization of Asp34 was detected in multiple sclerosis patients for both isoforms: HR(L-isoAsp)TGILDSIGR (p < 0.001, Mann–Whitney-U) and HR(D-isoAsp)TGILDSIGR (p = 0.002, Mann–Whitney-U). c An example of selected ion chromatographs from the tryptic digest of MBP from a control (66y) and a multiple sclerosis patient (48y). The percentage of modification was determined by the ion intensities of (HRDTGILDSIGR)/(HRDTGILDSIGR + HRDTGILDSIGR) × 100. Controls, n = 10; multiple sclerosis patients n = 8

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