Skip to main content

Table 7 Morphometric dendritic and dendritic spine parameters of LII-IIIPNs within the entorhinal cortex. All data presented as mean ± SEM

From: Marked changes in dendritic structure and spine density precede significant neuronal death in vulnerable cortical pyramidal neuron populations in the SOD1G93A mouse model of amyotrophic lateral sclerosis

Region

P8-15 (n) Neonatal

P28-35 (n) Pre-symptomatic

P65-75 (n) Onset

P120 (n) Mid-disease

Soma volume (μm3)

WT: 1172.4 ± 2198 (7)

WT: 1328.1 ± 1498 (5)

WT: 1162.2 ± 714 (5)

WT: 1132.7 ± 332.7 (8)

SOD1: 840.7 ± 1441 (5)

SOD1: 1045.4 ± 873 (5)

SOD1: 1232.2 ± 4599 (5)

SOD1: 1047.1 ± 409.5 (12)

Not significant

Not significant

Not significant

Not significant

Total arbor length (μm)

WT: 2691 ± 244 (7)

WT: 3166 ± 218 (5)

WT: 3732 ± 543 (5)

WT: 3150 ± 109 (8)

SOD1: 2229 ± 414 (5)

SOD1: 3375 ± 309 (5)

SOD1: 3145 ± 597 (5)

SOD1: 3235 ± 171 (12)

Not significant

Not significant

Not significant

Not significant

Apical arbor length (μm)

WT: 1197 ± 203 (7)

WT: 1754 ± 122 (5)

WT: 1881 ± 277 (5)

WT: 1651 ± 99 (8)

SOD1: 1123 ± 271 (5)

SOD1: 1515 ± 205 (5)

SOD1: 1481 ± 212 (5)

SOD1: 1636 ± 99 (12)

Not significant

Not significant

Not significant

Not significant

Basal arbor length (μm)

WT: 1493 ± 148 (7)

WT: 1394 ± 161 (5)

WT: 1831 ± 314 (5)

WT: 1506 ± 126 (8)

SOD1: 1105 ± 170 (5)

SOD1: 1859 ± 281 (5)

SOD1: 1657 ± 696 (5)

SOD1: 1548 ± 77 (12)

Not significant

Not significant

Not significant

Not significant

Mean basal tree length (μm)

WT: 501 ± 69 (7)

WT: 342 ± 14 (5)

WT: 400 ± 71 (5)

WT: 351 ± 24 (8)

SOD1: 408 ± 103 (5)

SOD1: 515 ± 51 (5)

SOD1: 348 ± 135 (5)

SOD1: 413 ± 24 (12)

Not significant

Not significant

Not significant

Not significant

Apical reach (μm)

WT: 543 ± 36 (7)

WT: 772 ± 74 (5)

WT: 619 ± 63 (5)

WT: 565 ± 13 (8)

SOD1: 520 ± 70 (5)

SOD1: 590 ± 45 (5)

SOD1: 760 ± 54 (5)

SOD1: 409 ± 38 (12)

Not significant

Not significant

Not significant

Not significant

Basal reach (μm)

WT: 166 ± 16 (7)

WT: 131 ± 6 (5)

WT: 173 ± 22 (5)

WT: 137 ± 9 (8)

SOD1: 136 ± 26 (5)

SOD1: 193 ± 11 (5)

SOD1: 126 ± 24 (5)

SOD1: 152 ± 17 (12)

Not significant

Not significant

Not significant

Not significant

Apical ramifications

WT: 5.3 ± 0.6 (7)

WT: 7.1 ± 0.6 (5)

WT: 7.4 ± 0.2 (5)

WT: 8.3 ± 1.6 (8)

SOD1: 6 ± 0.6 (5)

SOD1: 5.8 ± 0.8 (5)

SOD1: 8.9 ± 0.9 (5)

SOD1: 7.3 ± 0.2 (12)

Not significant

Not significant

Not significant

Not significant

Basal ramifications

WT: 6 ± 1.1 (7)

WT: 4.8 ± 0.3 (5)

WT: 4 ± 0.8 (5)

WT: 5 ± 0.3 (8)

SOD1: 5.8 ± 1.3 (5)

SOD1: 5 ± 0.5 (5)

SOD1: 3.5 ± 0.7 (5)

SOD1: 4.7 ± 0.2 (12)

Not significant

Not significant

Not significant

Not significant

Apical spine density per 100 μm

WT: 52.3 ± 7.2 (7)

WT: 39.8 ± 1.7 (5)

WT: 23.2 ± 2.2 (5)

WT: 28 ± 2.1 (8)

SOD1: 51.8 ± 8.8 (5)

SOD1: 39.6 ± 4.4 (5)

SOD1: 33.6 ± 4.9 (5)

SOD1: 35.7 ± 3.5 (12)

Not significant

Not significant

Not significant

Not significant

Basal spine density per 100 μm

WT: 55.6 ± 6 (7)

WT: 41.2 ± 3.7 (5)

WT: 23.4 ± 2.4 (5)

WT: 35.8 ± 3 (8)

SOD1: 60.6 ± 9.7 (5)

SOD1: 40 ± 3.4 (5)

SOD1: 35 ± 5.1 (5)

SOD1: 39.3 ± 2.9 (12)

Not significant

Not significant

Not significant

Not significant

  1. All analyses were unpaired two-way ANOVAs for age and genotype of LII-IIIPNs from the entorhinal cortex comparing soma volume (Age: P = 0.0010**; Genotype: P = 0.4552), total dendritic arbor length (Age: P = 0.0301*; Genotype: P = 0.4354), apical dendritic arbor length (Age: P = 0.0256*; Genotype: P = 0.9445), basal dendritic arbor length (Age: P = 0.0301*; Genotype: P = 0.4354), mean basal dendritic tree length (Age: P = 0.5982; Genotype: P = 0.6433), apical dendritic reach (Age: P = 0.0002***; Genotype: P = 0.1269), basal dendritic reach (Age: P = 0.7915; Genotype: P = 0.9994), apical branch ramifications (Age: P = 0.0139*; Genotype: P = 0.9351), basal branch ramifications (Age: P = 0.0742; Genotype: P = 0.7342), apical dendritic spine density per 100 μm (Age: P = 0.0001****; Genotype: P = 0.1906) and basal dendritic spine density per 100 μm (Age: P = 0.0002***; Genotype: P = 0.2384). Bonferroni post-tests were used to compare the effect of genotype at each age group, with the adjusted P-value (adj. P) reported in the table for parameters that had significant genotype effects. Each animal used (n) contains mean values from multiple individual neurons, specifically P8-15 (WT: 11 neurons; SOD1: 9 neurons), P28-35 (WT: 11 neurons; SOD1: 7 neurons), P65-75 (WT: 6 neurons; SOD1: 7 neurons) and P120 (WT: 13 neurons; SOD1: 14 neurons)
Back to article page

Acta Neuropathologica Communications

ISSN: 2051-5960

Contact us