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Table 1 Antibodies used in this study

From: Reduced Tau protein expression is associated with frontotemporal degeneration with progranulin mutation

Name Abbreviation Epitope Origin Provider Dilution Reference
Tau       
 Anti-total Tau (N-ter) N-ter First 19 aa in amino-terminal region Rabbit Home-made 1/10 000 [70]
 Anti-total- Tau (Tau 5) Tau 5 Middle region of Tau (aa 218–225) Mouse Invitrogen 1/2 000 [71]
 Anti-total-Tau (C-ter) C-ter Last 15 aa in carboxy-terminal region Rabbit Home-made 1/10 000 [72]
Synaptic proteins       
 α-synuclein α-syn Aa 15–123 of rat synuclein-1 Mouse BD Labsciences 1/500 [73]
 Post-synaptic density 95 PSD-95 Human PSD-95 Rabbit Cell Signaling 1/1000 [74]
 Munc-18 Munc-18 Aa 577–594 of rat Munc-18 Rabbit Sigma 1/10 000 [75]
 Synaptophysine SYP Aa 221–313 of human SYP Mouse Santa Cruz 1/10 000 [76]
Astrocytic proteins       
 Glutamine synthetase GS Aa 250–350 of Human GS Rabbit Abcam 1/10 000 N/A
 Glial Fibrillary Acidic Protein GFAP Bovin GFAP FL Mouse Santa Cruz 1/1000 [77]
Others       
 β-actin Actin N-ter Mouse Sigma-Aldrich 1/10 000 N/A
 Neuron Specific Enolase NSE Aa 269–286 of Human NSE Rabbit Enzo Life Science 1/50 000 N/A
 Aconitase   Bovine heart mitochondria Mouse Abcam 1/1000 [78]
 Histone H3 H3 C-terminus of human H3 Rabbit Millipore 1/10 000 [79]
  1. For each antibody, the full name, abbreviation, recognized sequence, origin, provider, dilution and literature reference are given. N/A Not Available