Table 1 Antibodies used in this study
Name | Abbreviation | Epitope | Origin | Provider | Dilution | Reference |
|---|---|---|---|---|---|---|
Tau | ||||||
Anti-total Tau (N-ter) | N-ter | First 19 aa in amino-terminal region | Rabbit | Home-made | 1/10 000 | [70] |
Anti-total- Tau (Tau 5) | Tau 5 | Middle region of Tau (aa 218–225) | Mouse | Invitrogen | 1/2 000 | [71] |
Anti-total-Tau (C-ter) | C-ter | Last 15 aa in carboxy-terminal region | Rabbit | Home-made | 1/10 000 | [72] |
Synaptic proteins | ||||||
α-synuclein | α-syn | Aa 15–123 of rat synuclein-1 | Mouse | BD Labsciences | 1/500 | [73] |
Post-synaptic density 95 | PSD-95 | Human PSD-95 | Rabbit | Cell Signaling | 1/1000 | [74] |
Munc-18 | Munc-18 | Aa 577–594 of rat Munc-18 | Rabbit | Sigma | 1/10 000 | [75] |
Synaptophysine | SYP | Aa 221–313 of human SYP | Mouse | Santa Cruz | 1/10 000 | [76] |
Astrocytic proteins | ||||||
Glutamine synthetase | GS | Aa 250–350 of Human GS | Rabbit | Abcam | 1/10 000 | N/A |
Glial Fibrillary Acidic Protein | GFAP | Bovin GFAP FL | Mouse | Santa Cruz | 1/1000 | [77] |
Others | ||||||
β-actin | Actin | N-ter | Mouse | Sigma-Aldrich | 1/10 000 | N/A |
Neuron Specific Enolase | NSE | Aa 269–286 of Human NSE | Rabbit | Enzo Life Science | 1/50 000 | N/A |
Aconitase | Bovine heart mitochondria | Mouse | Abcam | 1/1000 | [78] | |
Histone H3 | H3 | C-terminus of human H3 | Rabbit | Millipore | 1/10 000 | [79] |