Fig. 1

T5x mice exhibit robust accumulation of both beta- amyloid and tau pathologies within the hippocampus, cortex, and amygdala. Tau (Green; Dako total tau) and beta-amyloid (Red; 6E10) were examined by immunofluorescence and confocal microscopy in half brain coronal sections, revealing appropriate labeling of Aβ plaques and/or tau-laden neurofibrillary tangles in the hippocampus and overlying cortex of each genotype; WT (a), 5xfAD (b), Thy-Tau22 (c), T5x (d). Confocal settings were first established using T5x mice and then identical settings were used for all genotypes. Higher power images of the CA1 region of the hippocampus and amygdala further demonstrate a lack of either pathology in WT controls (e, i), Aβ pathology in 5xfAD mice (f, j), tau pathology in Thy-Tau22 mice (g, k), a combination of amyloid and tau pathology in T5x (h, l). Scale Bar = 300 μm in (a-d), 50 μm in (e-g), and 100 μm in (i-k)