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Table 2 GABAergic Interneurone Markers and Glutamatergic Markers in the Primary Visual Cortex in Dementia with Lewy Bodies and Alzheimer’s disease

From: Analysis of primary visual cortex in dementia with Lewy bodies indicates GABAergic involvement associated with recurrent complex visual hallucinations

Protein/mRNA Control DLB AD
GAD1 mRNA 1.46 ± 0.62 0.86 ± 0.22 0.62 ± 0.15*
GAD67 protein 0.85 ± 0.21 0.85 ± 0.16 0.72 ± 0.13
GAD65 protein 1.12 ± 0.26 1.16 ± 0.26 0.91 ± 0.15
PVALB mRNA 1.59 ± 0.47 0.87 ± 0.24† 3.51 ± 0.55**
PVALB protein 0.70 ± 0.22 0.61 ± 0.25* 0.87 ± 0.25***
NPY pg/mg 5.71 ± 1.95 6.63 ± 1.62 7.39 ± 1.52*
SST ng/mg 0.42 ± 0.18 0.48 ± 0.15 0.60 ± 0.27†
GABARAP-17 kDa 1.56 ± 0.28 0.91 ± 0.19*** 1.29 ± 0.28**
GABARAP-14 kDa 1.47 ± 0.27 0.99 ± 0.14*** 1.15 ± 0.26**
Gephyrin 1.13 ± 0.27 0.84 ± 0.08** 0.67 ± 0.09**
GABA A α1 0.75 ± 0.30 0.82 ± 0.26 0.83 ± 0.39
Kif5A 0.95 ± 0.58 0.51 ± 0.10† 0.90 ± 0.09
GRIN2A mRNA 1.44 ± 0.79 0.70 ± 0.16 0.89 ± 0.31
GluR1/GRIA1 protein 0.94 ± 0.13 0.89 ± 0.33 NT
vGlut1(SLC17A7) protein 61.3 kDa 1.20 ± 0.24 1.04 ± 0.18 0.91 ± 0.11†
vGlut1(SLC17A7) protein 59.3 kDa 0.90 ± 0.13 0.87 ± 0.1 0.90 ± 0.12
PSD95 (DLG4) mRNA 3.33 ± 1.14 1.80 ± 0.61† 0.83 ± 0.26*
PSD-95 protein 1.25 ± 0.22 0.62 ± 0.19*** 1.24 ± 0.28
GAP43 protein 1.25 ± 0.45 0.71 ± 0.22*** 1.08 ± 0.16
BDNF mRNA 0.70 ± 0.16 0.39 ± 0.14* 0.71 ± 0.48
BDNF protein ND ND ND
Synaptophysin (SYP) mRNA 1.76 ± 0.72 0.89 ± 0.22 2.69 ± 0.80
Synaptophysin protein 0.96 ± 0.25 0.54 ± 0.09*** 0.72 0.16*
SNAP25 mRNA 1.27 ± 0.30 0.88 ± 0.23 0.83 ± 0.25
SNAP25 protein 1.10 ± 0.28 0.99 ± 0.13 1.10 ± 0.17
  1. Relative expression levels (+/- SEM) for GABAergic interneurone marker transcripts and proteins in control, DLB and AD primary visual cortex were determined using validated Taqman assays by real time PCR and proteins determined using western blotting of the appropriate protein band with relative expression levels being normalised to GAPDH mRNA or protein. Values represent the 2-ΔΔCT value for mRNA and protein/GAPDH for the specific protein. NPY and SST levels were determined using specific sandwich ELISA. Uncorrected p values are presented with, *, P < 0.05 relative to control; **, P < 0.01 relative to control; ***, P < 0.005 relative to control; †, P < 0.1, >0.05. BDNF protein was not detected (ND) in any samples using an ELISA method
  2. NT Not tested