Fig. 1

Characterization of Notch1 expression in sporadic AD brain tissue. a–b’) Immunohistochemical analysis on hippocampus (a–a’) and entorhinal cortex (b–b’) shows an aberrant Notch1 deposition in the brain parenchyma. Moreover, in AD patients, Notch1 labeling appears fainter in the cell bodies and processes of cortical and hippocampal neurons as compared to healthy controls (a–b). a’-b’) Notch1 positive plaques with different conformation either core plaques (upper captions) or diffuse distribution (bottom captions). c–d) Box plots summarizing the quantification of Notch1 positive pixels occupying the soma of (c) hippocampal and (d) cortical neurons of AD and healthy control patients (p<0.001 for both regions). e) Double immunofluorescence analysis on the dorsal hippocampus indicates that, in AD tissue, Notch1 is deposited in A β42 positive plaques and is decreased in neuronal soma as compared to healthy controls. Nuclei have been counterstained using DAPI. f) Bar graph showing the counting of plaques/100 μ m ², in the hippocampus, positive for Notch1, A β42 and double positive. g) Bar graph showing the counting of plaques per 100 μ m ², in the entorhinal cortex, positive for Notch1, A β42 and double positive. *= p<0.05, **= p<0.01. Error bars are SEM. Scale bars are 100 μm in (a) and (e), and 25 μm in (a’) and (b’)