Fig. 1

Features of 993 differentially methylated CpG sites in pilocytic and diffuse astrocytomas compared to controls. a Three-way comparison of differentially methylated sites in pilocytic astrocytomas, diffuse astrocytomas and control brain tissue. b Numbers of common and unique differentially methylated sites in pilocytic and diffuse astrocytomas. c Percentages of hypermethylated (dark grey) and hypomethylated (light grey) differentially methylated sites in pilocytic and diffuse astrocytomas compared to normal controls. d Hierarchical clustered heatmap showing 993 differentially methylated sites. Methylation beta values are represented in shades of blue = 0 (methylated) to yellow = 1 (unmethylated). Tumour type, tumour location, age, sex, KIAA1549-BRAF fusion status, and genomic mutations are shown [65]. FFL, Foetal frontal lobe; Foetal, foetal brain; FCB, foetal cerebellum; Adult, adult brain, NSC, neural stem cells; PA, pilocytic astrocytomas; DA, diffuse astrocytomas; ND, not determined. e Diagrammatic representation of the Illumina 450K annotated CpG features in relation to intragenic locations, and f in relation to UCSC Genome Browser-annotated CpG islands. g Distribution of the differentially methylated CpG sites across the annotated gene regions. TSS1500, 201-1500 bp upstream of transcription start (TSS); TSS200, up to 200 bp upstream of the TSS; UTR, untranslated region. h Distribution of the differentially methylated CpG sites in relation to CpG islands. i Distribution of the differentially methylated CpG sites in relation to CpG density [42]. HC, high CpG density; IC, intermediate CpG density; IC shore, intermediate CpG density shore; LC, low CpG density