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Table 1 Primers used for site-directed mutagenesis

From: Critical residues involved in tau binding to fyn: implications for tau phosphorylation in Alzheimer’s disease

Primer name

Primer sequence 5′ to 3′

Forward P176A

AGCAAAAACCGCTCCCGCTCCAAAG

Reverse P176A

CTTTGGAGCGGGAGCGGTTTTTGCT

Forward P182A

CGCTCCAAAGACAGCACCCAGCTCTGG

Reverse P182A

CCAGAGCTGGGTGCTGTCTTTGGAGCG

Forward P200A

TACAGCAGCGCAGGCTCTCCAGGCACTC

Reverse P200A

GAGTGCCTGGAGAGCCTGCGCTGCTGTA

Forward P206A

GGCTCCCCAGGCACTGCCGGCAGC

Reverse P206A

GCTGCCGGCAGTGCCTGGGGAGCC

Forward P213A

GCTCCCGCACCGCGTCCCTTCCA

Reverse P213A

TGGAAGGGACGCGGTGCGGGAGC

Forward P219A

CCCTTCCAACCCCAGCCACCCGGG

Reverse P219A

CCCGGGTGGCTGGGGTTGGAAGGG

  1. Primers used for site-directed mutagenesis of tau. The codons mutated to replace proline (P) with alanine (A) in the encoded protein are underlined
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Acta Neuropathologica Communications

ISSN: 2051-5960

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