Fig. 3

Correlative autoradiography (ARG) and immunohistochemical (IHC) findings for assessment of correlative binding in typical Alzheimer’s disease (AD, case 7). AV-1451 ARG (AV-1451 blocking shown in the left ARG inset), PHF-1 and CP13 immunohistochemistry in the posterior hippocampal region in a Braak VI, Thal 5 AD case. Tau pathology in the subiculum is shown in the upper right inset (black arrowhead) and shows very good signal on AV-1451 ARG, PHF-1 and CP13. However, the pattern of AV-1451 binding is similar but not identical to IHC with some focal discordance in many regions including Sommer’s sector of the hippocampus where CA1 and subiculum meet, the superficial layers of the parahippocampal and occipitotemporal gyri (*). Intense, linear AV-1451 signal in cortex matches best with the internal pyramidal cell layer (5) (right lower inset) while superficial layers (1-4) have more minimal-moderate AV-1451 signal where there is intense IHC positivity (right lower inset and yellow arrow). Scale bar for all full size hippocampus at 5 mm, and for 20x zoomed insets at 25 μm. Black arrowheads indicate 20x inset locations