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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Neuronal accumulation of unrepaired DNA in a novel specific chromatin domain: structural, molecular and transcriptional characterization

Fig. 6

a–e Confocal microscopy images of SGN nuclei co-stained for γH2AX in combination with fibrillarin (a, b), coilin (c), histone H4K20me3 (d) and HP1γ (e). They illustrate the direct association of PDDF with a triad of structures: the nucleolus (a, c, e), Cajal bodies, immunolabeled for fibrillarin (b) or coilin (c), and perinucleolar heterochromatin clumps immunolabled for the tri-methylated histone H4K20me3 (d) or HP1γ (e). f Double immunolabeling for 53BP1 and TMG-cap illustrate the distribution of the nuclear speckles of splicing factors labelled with the anti-TMG-cap antibody and the close association of a PDDF with a speckle in the vicinity of the nucleolus. 45d post-IR. Scale bar = 5 μm

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