Skip to main content
Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Neuronal accumulation of unrepaired DNA in a novel specific chromatin domain: structural, molecular and transcriptional characterization

Fig. 5

Immunogold electron microscopy transcription assay based on the incorporation of 5’-FU into nascent RNA after 45 min of the intraperitoneal administration of 5’-FU. 45d post-IR. a This PDDF (asterisk) lack of immunogold labeling indicating the absence of transcription. However, the euchromatin, including de domains flanking the PDDF appeared intensely labeled with gold particles of 5’-FU incorporation. Scale bar: 300 nm. b Detailed of 5’-FU incorporation in the euchromatin boundary of a PDDF (asterisk). Scale bar: 200 nm. c Detail of a PDDF (asterisk) associated with both the nucleolus (No) and a Cajal Body (CB). Note the absence of nascent RNA in the PDDF and in the transcription-free CB, whereas the nucleolus and adjacent euchromatin appeared immunogold labeled. d Immunogold electron microscopy for the active RNA polymerase II shows the absence of labeling in a PDDF (asterisk), whereas the surrounding euchromatin appeared decorated with gold particles Scale bar: 150 nm

Back to article page