Fig. 7
From: Fasudil attenuates aggregation of α-synuclein in models of Parkinson’s disease
Fasudil treatment reduces the number of cells with α-Syn-aggregates without altering neuronal numbers in the midbrain of α-SynA53T mice. a. Immunohistochemical staining against proteinase-K resistant α-Syn aggregates, as well as NeuN, TH and TPH2 in combination with nuclear staining (DAPI) was performed to investigate the effect of Fasudil on α-Syn pathology in α-SynA53T mice after disease onset. Treatment groups were wildtype control (wt ctrl), α-SynA53T control (A53T ctrl), α-SynA53T with 10 mg/kg bw Fasudil (A53T Fas10) and α-SynA53T with 30 mg/kg bw Fasudil (A53T Fas30). b. Immunohistochemical analysis of red nucleus (RN; n(wt ctrl) = 5, n(A53T ctrl) = 7, n(A53T Fas10) = 6, n(A53T Fas30) = 6 for α-Syn aggregates; n(wt ctrl) = 6, n(A53T ctrl) = 6, n(A53T Fas10) = 6, n(A53T Fas30) = 6 for NeuN. c. Immunohistochemical analysis of substantia nigra (SN; n(wt ctrl) = 5, n(A53T ctrl) = 8, n(A53T Fas10) = 8, n(A53T Fas30) = 9 for α-Syn aggregates; n(wt ctrl) = 5, n(A53T ctrl) = 6, n(A53T Fas10) = 6, n(A53T Fas30) = 6 for TH. d Immunohistochemical analysis of the dorsal raphe nucleus (DR; n(wt ctrl) = 4, n(A53T ctrl) = 4, n(A53T Fas10) = 9, n(A53T Fas30) = 5 for α-Syn aggregates; n(wt ctrl) = 4, n(A53T ctrl) = 4, n(A53T Fas10) = 9, n(A53T Fas30) = 5 for TH. Drawings on the left show localization of respective brain regions, exemplary pictures in the middle show cells from respective brain regions with proteinase-K resistant α-Syn aggregates, as well as NeuN, TH, TPH2 and DAPI positive cells in brains of untreated α-SynA53T mice. Data on the right are presented as means ± SEM; * p <0.05; ** p < 0.01; *** p < 0.001; n.s. = not significant; T-Test for comparisons between untreated groups; ANOVA with Dunnett post-hoc test for multiple group comparisons between α-SynA53T groups; 1M px2 = one million square pixels. Scale bar = 20 μm