Fig. 5

Clobetasol promotes remyelination in NMO. a. Mice were treated with clobetasol or vehicle starting on day 4 and sacrificed at day 9. One group of mice were sacrificed at day 4 without treatment (‘non-treated’ mice). b. Immunofluorescence of AQP4 and MBP from non-treated (day 4), vehicle-treated (day 9) and clobetasol-treated (day 9) mice; arrows point to site of infusion (left). Areas of loss of AQP4 and MBP immunofluorescence normalized with brain hemisphere area (mean ± S.E., 3 mice per group, ** P < 0.01) (right). c. Immunofluorescence for indicated markers. d. Mice were treated with dexamethasone (DEX) or vehicle starting on day 4 and sacrificed at day 9. e. Representative immunofluorescence (left) with quantitative data for loss of AQP4 and MBP immunofluorescence (mean ± S.E., 3 mice per group, differences not significant) (right)