Fig. 8

Insoluble proteins form NFT-like aggregates rapidly after OA injection at both the injection site and distal brain regions. a β-pleated sheet protein structures are identified by thioflavin-S. Intracellular aggregates are observed at both the injection site and distal sites in the somatosensory cortex at 24 h, which persist 7 days post injection. Scale bar = 50 μM. b High magnification of thioflavin-S positive cells in the amygdala and somatosensory cortex. Scale bar = 10 μm. c Coronal sections taken from OA injected MAPT KO show no phospho-tau immunoreactivity. d No thioflavin positive structures are observed in MAPT KO mice with background staining comparable to the wild-type controls at 24 h. Scale bar = 50 μm