Fig. 5
From: High expression of α-synuclein in damaged mitochondria with PLA2G6 dysfunction
Prominent formation of Lewy bodies in PLAN. a Parkinson’s disease (PD) case; b-f PLA2G6-associated neurodegeneration (PLAN) case; a, b Dorsal motor nuclei of vagus (nX); c, d Locus coeruleus (LC); e, f Substantia nigra (SN); a-c, e Immunohistochemistry for PαSyn plus PAS staining; d, f Double immunohistochemistry for PαSyn (brown) and tyrosine hydroxylase (TH, blue). Scale bars represent 40 μm in (a-c, e), and 20 μm in (d, f). a In PD, PαSyn-positive inclusions, Lewy bodies (arrows) are observed mainly in the neuropil. b In PLAN, there are many Lewy neurites and Lewy bodies (LBs, arrows) both in the neurons and in the neuropil. c Cytoplasmic LBs are indicated with arrows. d Spherical Lewy neurites in the center of this panel and a LB in TH-positive neuron (arrow) are shown. e The neurons in the locus coeruleus are well preserved despite prominent LBs (arrows). f The LBs in TH-positive neurons are small, but have the apparent core and halo structure (arrows)