Fig. 5
From: Super-resolution microscopy reveals γ-secretase at both sides of the neuronal synapse
Localization of γ-secretase at the postsynaptic plasma membrane. (a) STED images showing localization of γ-secretase (red) very close to the synaptic cleft in the dendritic spines (white arrows). Synaptophysin (blue) and PSD95 (green) mark the pre- and postsynaptic sides, respectively. The close-ups are taken from Additional file 1: Figure S5b (a1) and S5d (a2). (b) STORM imaging of γ-secretase (red) and NMDAR2B (green). The image shows a typical STORM image on a brightfield overlay (left), with increased magnification in the middle and right panels, displaying a close-up from one individual postsynaptic membrane region to the right. (c) The same postsynaptic membrane region as the right image in (b) is displayed in 3D view from three different angles where red, green and blue lines represent X, Y and Z directions, respectively