Fig. 3
From: Super-resolution microscopy reveals γ-secretase at both sides of the neuronal synapse
STED imaging of γ-secretase in synapses with triple-staining. γ-Secretase and the synaptic markers were visualized to show the synaptic location of γ-secretase together with f-actin to outline the cellular structure. (a) Localization of γ-secretase (red) at the synapse using synaptophysin (blue) as a presynaptic marker. Dendritic spine structure was visualized with Phalloidin as a marker for f-actin (white). The distribution of γ-secretase throughout the synaptophysin-stained region is marked by the upper arrow, whereas the γ-secretase staining in the dendritic spine is shown by the lower arrow. (b) Localization of γ-secretase (red) at the synapse using PSD95 (green) as a postsynaptic marker and phalloidin as a marker for f-actin (white). The presence of γ-secretase in the postsynaptic compartment is shown, where the postsynaptic marker PSD95 is found in dense regions inside the dendritic spine head (arrow)