Fig. 4

Virus-mediated delivery of NRG1 extends the survival time of SOD1^G93A mice. (a) Semi-quantitative reverse transcription PCR for Type III-NRG1 and β-actin mRNA in AAV-Type III-NRG1 injected- and AAV-GFP injected- spinal cords. This PCR reaction amplifies endogenous NRG1 as well (GFP). (b, c) Kaplan-Meier curve of onset (c) and survival time (b) for SOD1^G93A mice injected with AAV-Type III-NRG1 (red) (survival time: male = 3, female = 8, onset: male = 5, female = 10), AAV-Type I-NRG1 (blue) (survival time: male = 4, female = 6, onset: male = 6, female = 8), or AAV-GFP (black) (survival time: male = 4, female = 7, onset: male = 6, female = 9). (d, e) Mean survival (d) and onset time (e) of SOD1^G93A mice injected with indicated virus are plotted. Error bars denote SD. **: p < 0.05 (Gehan-Breslow-Wilcoxon test)