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Table 3 Transmission of MM2-sCJD prions to ovine PrP mice after PMCA amplification or passage through P2FJ6 cells

From: Emergence of two prion subtypes in ovine PrP transgenic mice infected with human MM2-cortical Creutzfeldt-Jakob disease prions

Inoculum

Passage

Mean incubation timea (n/n0)

PrPres patternb

   

Brain

Spleen

MM2-sCJD

5

80 ± 1 (6/6)

T2Ov

T1Ov

Cloned MM2-sCJDc

2

79 ± 1 (6/6)

T2Ov

T2Ov

MM2-sCJD → PMCA

1

105 ± 1 (5/5)

T1Ov

T1Ov

MM2-sCJD → PMCA → tg338 brain

2

84 ± 2 (6/6)

T1Ov

T1Ov

MM2-sCJD → PMCA → tg338 spleen

2

93 ± 2 (6/6)

T1Ov

T1Ov

MM2-sCJD → P2FJ6 cells

1

80 ± 1 (6/6)

T2Ov

T1Ov

Cloned MM2-sCJD → P2FJ6 cells

1

75 ± 2 (6/6)

T2Ov

neg

MM2-sCJD → PMCA → P2FJ6 cells

1

118 ± 1 (6/6)

T1Ov

T1Ov

MM2-sCJD → RK13 cells → tg338

1

> 400 d (0/6)

neg

neg

  1. n/n0: number of mice with neurological disease and positive for PrPres in the brain by immunoblotting/number of inoculated mice
  2. aDays ± SE of the mean.
  3. b T1Ov and T2Ov refers to the migration pattern of unglycosylated PrPres at ≈ 21 kDa and ≈ 19 kDa (in the brain), respectively.
  4. ctg338-MM2-sCJD prions were cloned by end-point titration in reporter tg338 mice and sub-passed.
  5. Brain was used for inoculation unless mentioned
  6. Neg: negative
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Acta Neuropathologica Communications

ISSN: 2051-5960

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