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Table 3 Transmission of MM2-sCJD prions to ovine PrP mice after PMCA amplification or passage through P2FJ6 cells

From: Emergence of two prion subtypes in ovine PrP transgenic mice infected with human MM2-cortical Creutzfeldt-Jakob disease prions

Inoculum Passage Mean incubation timea (n/n0) PrPres patternb
    Brain Spleen
MM2-sCJD 5 80 ± 1 (6/6) T2Ov T1Ov
Cloned MM2-sCJDc 2 79 ± 1 (6/6) T2Ov T2Ov
MM2-sCJD → PMCA 1 105 ± 1 (5/5) T1Ov T1Ov
MM2-sCJD → PMCA → tg338 brain 2 84 ± 2 (6/6) T1Ov T1Ov
MM2-sCJD → PMCA → tg338 spleen 2 93 ± 2 (6/6) T1Ov T1Ov
MM2-sCJD → P2FJ6 cells 1 80 ± 1 (6/6) T2Ov T1Ov
Cloned MM2-sCJD → P2FJ6 cells 1 75 ± 2 (6/6) T2Ov neg
MM2-sCJD → PMCA → P2FJ6 cells 1 118 ± 1 (6/6) T1Ov T1Ov
MM2-sCJD → RK13 cells → tg338 1 > 400 d (0/6) neg neg
  1. n/n0: number of mice with neurological disease and positive for PrPres in the brain by immunoblotting/number of inoculated mice
  2. aDays ± SE of the mean.
  3. b T1Ov and T2Ov refers to the migration pattern of unglycosylated PrPres at ≈ 21 kDa and ≈ 19 kDa (in the brain), respectively.
  4. ctg338-MM2-sCJD prions were cloned by end-point titration in reporter tg338 mice and sub-passed.
  5. Brain was used for inoculation unless mentioned
  6. Neg: negative