Fig. 3
Expression of hTau40AT induces pathological hyperphosphorylation and conformational changes of Tau in 3–5 months old hTau40AT mice. a Illustration of human full length Tau with highlighted phospho-Tau antibody-epitopes AT180 (pThr231/pSer235), AT8 (pSer202/pThr205), 12E8 (pS262/pS356) and PHF1 (pSer396/pSer404) and pathological Tau conformation recognized by antibody Alz-50 (aa 7–9 + 312–342). b-f Hyperphosphorylated, mislocalized and conformationally changed Tau in brain and spinal cord of 3–5 months old hTau40AT mice detected by antibodies: (b) 12E8, (c) AT180, (d) AT8, (e) PHF1 and (f) Alz-50. Note Tau missorting into the somatodendritic compartment of hippocampal, cortical and spinal neurons (arrows). Asterisks indicate localization of phosphorylated and conformationally changed Tau in the stratum lucidum. In WT mice, areas CA3, cortex and spinal cord are non-reactive for 12E8, AT180, AT8 and PHF1 (b-e1, b-e3, b-e5). Pathological Tau conformation identified by Alz-50 antibody in brain (f2 (asterisk = CA3-mossy fibers), f4) and spinal cord (f6) of hTau40AT mice compared to WT littermates (f1, f3, f5). WT: wild type; A152T: hTau40AT transgenic mouse strain; Ctx: cortex; SpC: spinal cord; CA: cornu ammonis; Scale bar: 50 μm (b1-f6)