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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Low autophagy capacity implicated in motor system vulnerability to mutant superoxide dismutase

Fig. 1

Autophagy factors are present in exceptionally low amounts in spinal ventral horns of human controls. a, b Western blots for autophagic proteins in human postmortem specimens of distinct CNS regions from (a) non-neurological and (b) neurodegenerative (Parkinson’s disease; PD) controls. Aliquots of protein extracts (20 μg) were loaded in each well. β-Actin was used as a loading marker. To allow multiple comparisons between different gels, grey matter from the anterior 3rd of the temporal superior gyrus from a non-neurological control was used as a standard. Triplicate analyses of the proteins were made. Analysis of multiple triplicates indicated a relative standard deviation of the estimates of around 7 %. cf Scatter plots showing the expression patterns of autophagic proteins including (c) Beclin 1, (d) Atg12 (detected as Atg12-Atg5 complex), (e) LC3-II, and (f) p62. All values in each CNS region were normalized to the level of expression of the standard. Bars represent mean values. *P < 0.05 vs. ventral horn of non-neurological controls. **P < 0.01 vs. ventral horn of non-neurological controls. ## P < 0.01 vs. ventral horn of PD (one-way ANOVA with Tukey-Kramer’s test.)

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