Fig. 2
Gender influences O2 consumption in lumbar spinal cord slices from hSOD-G93A mice. Graphs shows baseline (a), complex I (b), complex II (c), and residual (d) O2 consumption in slices from male and female G93A mice. Measurements were taken at 37 °C in Hank’s balanced salt solution containing 10 mM Hepes, pH 7.4, and corrected for instrumental background O2 flux. After permeabilization, similar measures were performed for endogenous (e), complex I state 2 (f), complex I state 3 (g), complex I/II state 3 (h) and complex II state3 (i) O2 consumption. Measurements were taken at 37 °C in Mir05 media. Values shown are means ± SEM expressed as pmol O2/min · mg protein for 4-6 independent measures. *, p < 0.01 with respect to females of the same age with Student’s t test