Fig. 5

Phospho-ERK analysis. a Immunohistochemistry on section from case 1 showing a strong expression of phospho-ERK. Magnification x200. b Total lysates from individual tumors (case 1, 2 and 3) were subjected to western blotting for expression analyses of phosphorylated (p-) and non-phosphorylated ERK1/2. Pilocytic astrocytoma expressing KIAA1549-BRAF fusion was used as positive control (C+). ß-actin was used as reference