Fig. 1
Clinically intensive exacerbation in the E5415A model. No significant (NS) clinical exacerbation occurred in the hIgGNMO model (a). In contrast, acute and severe exacerbation was observed in rats receiving 0.1–1 mg E5415A, in an IgG-dose-dependent manner (b). Within 48 h after the IgG injection, the clinical disability score in the 1 mg E5415A models was statistically significant compared with those in the 0.1 mg (p = 0.0413), 0.01 mg (p = 0.0272), normal EAE (p = 0.0101), and hIgGcont groups (p = 0.0038). In addition, the score in the 0.1 mg E5415A rats was also significantly higher than that in the hIgGcont rats (p = 0.0280). These changes strikingly occurred at 6–12 h after the NMO-IgG injection. Within 6–12 h, the disability score in the 1 mg E5415A model was statistically significantly different to those in the 0.1 mg (p = 0.0209), 0.01 mg (p = 0.0376), normal EAE (p = 0.0160), and hIgGcont groups (p = 0.0057), and the score in the 0.1 mg group was significantly higher than that in the hIgGcont group (p = 0.0284)