Fig. 8

Brains of mice injected with brain extracts from MSA and probable iLBD cases but not PBS accumulate phosphorylated alpha-synuclein. Brains of Tg(SNCA)1Nbm/J mice injected with brain extracts from MSA or probable iLBD cases or PBS were biochemically analyzed at 9 months post injection with antibodies against alpha-synuclein (Syn211) or phosphorylated alpha-synuclein (81A). All mouse brains contained equal amounts of sarkosyl-soluble alpha-synuclein (upper panel). Phosphorylated alpha-synuclein, however, was not detectable in mouse brains injected with PBS but in mouse brains injected with brain extracts from MSA or probable iLBD cases (lower panel). Detection of tubulin beta chain shows equal loading in all lanes. Molecular sizes are shown in kilodalton