Fig. 3

Biochemical and histological analysis of alpha-synuclein expression in wild-type and Tg(SNCA)1Nbm/J mice. Biochemical analysis with an antibody against alpha-synuclein (clone 42) that equally reacts with human as well as mouse alpha-synuclein shows that human alpha-synuclein is only very moderately overexpressed in Tg(SNCA)1Nbm/J mice in comparison to alpha-synuclein expression in wild-type mice. Equal amounts of total protein (5 μg) were loaded onto each lane of the SDS-polyacrylamide gel and show expression of alpha-synuclein in the striatum (Str), cortex (Ctx), hippocampus (Hc), brainstem (Bs), and cerebellum (Cb). Molecular sizes are shown in kilodalton a. The signal for alpha-synuclein was quantified by densitometry from western blots and normalized against tubulin and is shown as fold overexpression of alpha-synuclein in Tg(SNCA)1Nbm/J mice (n = 3) versus wild-type mice (n = 3) b. Immunohistochemistry of mouse brain sections with the same antibody (clone 42) against alpha-synuclein reveals comparable localization of alpha-synuclein in Tg(SNCA)1Nbm/J and wild-type mice c