Fig. 6

Analysis of amyloid distribution and morphology in tet.MoAβ(GFP) mice. The overall distribution of deposited amyloid is shown by digital extraction of staining by Cambell-Switzer silver staining. Additional representative images of cortical amyloid in tissue sections stained with antibodies to GFAP, Aβ (mAb 4G8 and rAb Aβ42) and ubiquitin, and by Campbell-Switzer silver stain or Thioflavin-S staining. The images shown are representative of images from an analysis of at least 3 animals at each age and each genotype with at least 6 individual tissue sections taken from similar levels for each animal. The example of Thioflavin-S staining of the tet.MoAβ(GFP) tissue represents the maximal fluorescent intensity that was observed. All the images were shown at the same amplification after cropping from 40x microscope images