Fig. 3
From: Two alternative pathways for generating transmissible prion disease de novo
Analysis of brain materials from the first passage. a Western blots of brain materials from animals inoculated with F0.5-induced atypical PrPres and sacrificed at 441 days post-inoculation. Brain material from animal inoculated directly with F0.5 is included as a reference. b Schematic representation of the PK resistance profile showing overlap between atypical PrPres and PrPSc, where atypical PrPres and PrPSc are represented by gray and black boxes, respectively. c Western blot analysis of PK-resistant materials amplified in serial PMCAb reactions seeded with brain materials from animals inoculated with F0.5-induced atypical PrPres and sacrificed at 441 days. d Western blots of brain materials from animals inoculated with F0.5 fibrils, F0.5 fibril-induced atypical PrPres, 0.3 % S05 brain material or brain-derived atypical PrPres prepared as described in Fig. 2c. Western blots were stained with SAF-84 or 3F4 antibody as indicated