Table 2 Primary antibody information
Western Blots | ||||
ANTIBODY | COMPANY | CODE | Dilution | |
PHF1 | Peter Davies | 1:500 | ||
Tau13 | Covance | MMS-520R-500 | 1:2000 | |
GAPDH | Abcam, | Ab8245 | 1:2000 | |
Beta-actin | Abcam | Ab8226 | 1:2000 | |
Synaptophysin | Abcam | Ab8049 | 1:5000 | |
Beta-III-tubulin | Abcam | Ab18207 | 1:1000 | |
MBP | AbD Serotec | MCA409s | 1:500 | |
Histone | Abcam | Ab1791 | 1:1000 | |
VDAC1/Porin | Abcam | Ab34726 | 1:500 | |
GluN2B | BD Biosciences | 610416 | 1:500 | |
Synapsin | Millipore | AB1543P | 1:20000 | |
Neuropathology | ||||
ANTIBODY | COMPANY | CODE | Dilution | Pre-treatment |
Beta Amyloid (BA4) | Dako | M087201-2 | 1:100 | 98% formic acid 5 min |
Alpha Synuclein | Life Technologies | 32-8100 | 1:200 | Pressure cooker/formic acid |
TDP-43 | 2B Scientific | CAC-TIP-PTD-MO1 | 1:4000 | Pressure cooker/citric acid |
pTau (AT8) | Thermo | MN1020 | 1:2500 | None |
Ubiquitin | Dako | Z0458 | 1:500 | Pressure cooker/citric acid |
GFAP | Dako | Z0334 | 1:800 | None |
CD68 | Dako | M0876 | 1:100 | Pressure cooker/citric acid |
Array Tomography | ||||
ANTIBODY | COMPANY | CODE | Dilution | Secondary Antibody |
AW7 | Dominic Walsh | 1:1000 | Donkey α Rabbit – AF488 | |
Synaptophysin | Abcam | Ab8049 | 1:50 | Donkey α Mouse – AF594 |
ApoE | Abcam | Ab7620 | 1:50 | Donkey α Goat – AF647 |
PSD95 | Abcam | Ac12093 | 1:50 | Donkey α Goat – AF488 |