Using array tomography to assess the presence of synaptotoxic proteins. Representative images from a single region of interest (crop) captured within the LBC1936 EC (A+C) or the AD EC (B+D). Each image is a single plane from a 3D stack, which has been thresholded/binarised and single-slice objects removed to eliminate background. Sections were stained for synaptophysin, PSD95, and AW7 (A+B) or ApoE (C+D). Synaptically located staining is highlighted with white circles. C. Scale bar = 2μm.