Fig. 7

Regional swelling after MCAO. a image of a coronal slice of brain tissue from a rat submitted to 4.5 hour ischemia and 24 hours of reperfusion, stained with the myelin stain Black Gold II and counterstained with cresyl violet, demonstrating the raw image data prior to volumetric analysis; lateral corpus callosum (filled arrowhead), cortex (empty arrowhead). b hue channel obtained from red-green-blue to hue-saturation-intensity transformation of A, demonstrating the input image to the algorithm used for automated segmentation of subcortical white matter. c image of output from active contour segmentation of B showing ipsilateral and contralateral cortex segmentations (green) and subcortical white matter segmentations (red) overlaid with the input hue image in B. d visualization of quantification of hemispheric swelling; the ordinate reflects the combination of the ipsilateral (Ipsi.) or contralateral (Contra.) segmentations normalized to the combination of the contralateral segmentations; n = 5 rats per group; * p < 0.05 comparison to the contralateral baseline, depicted as dotted horizontal line. e visualization of quantification of swelling of cortex (Grey) and subcortical white matter (White); the ordinate reflects the volume of either the ipsilateral cortical grey matter or ipsilateral subcortical white matter normalized to the volume of the contralateral cortex or subcortical white matter; n = 5 rats per group; * p < 0.05 comparison to the contralateral baseline, depicted as dotted horizontal line; # p < 0.05 comparison between cortical grey matter versus subcortical white matter