Fig. 5
From: C9ORF72 expression and cellular localization over mouse development
Expression of C9ORF72 in synaptosome preparations from mouse brains. Figure shows representative western blots with results from 2 animals for each marker (indicated by 1 and 2 on figure). The 55 kDa isoform of C9ORF72 was present in the combined F3/F4 fractions which contain synaptosomes. C9ORF72 expression was low in fraction F1, containing membranes, and F2 containing myelin, membranes and glia. Unfractionated brain at P56 was also included. Purity of fractions was determined by labeling with GFAP (F2) and synaptic markers, synaptophysin, PSD-95 and GAD67 (F3/F4)