Fig. 4

Transferred lymphocytes infiltrate the retina and spleen. a, b Immunohistochemical detection of DsRed/CD3⁺ T-lymphocytes (red) derived from (a) healthy B6 (b) nee donors in the retina of recipient mice 20 days after transfer. The presence of sporadic extravasated DsRed⁺/CD3⁺ T-lymphocytes located either within the recipients’ RGC layer (shown in a) or epiretinally on the surface of the nerve fiber layer (shown in b) was noted in all recipients. V = retinal vessel. c-f Infiltration of transferred DsRed⁺ lymphocytes into the spleen of recipient animals 28 days after injection. Integration of immune cells obtained from nee donors was noticeably more pronounced than that of those derived from B6 donors. Notation of CD3 or CD19 in the microphotographs refers to the transferred lymphocyte fraction rather than immunohistochemical detection. DAPI was used to label nuclei