Fig. 5

ChABC injection increases density of synaptophysin surrounding Aβ plaques in the slm. a a. freehand rings were drawn around three regions of an amyloid plaque (inner, middle and outer). a b. rings were transferred to the identical location on the synaptophysin image and density measured in each ring. a c. identically drawn rings were moved to an adjacent, non-plaque region and synaptophysin density measured. b. quantitative density measurements of synaptophysin immunoreactivity around plaques in slm of 15 months old APPswe/PS1dE9 mice. Measurements were made in the slm after ChABC injection on the (c) contralateral, non-injected hemisphere and the (d) injected, ipsilateral side. The y-axis term “Proportion of non-plaque density” refers to the ratio of synaptophysin density of the plaque region divided by the synaptophysin density of the non-plaque region. This proportion was calculated for the “inner”, “middle” and “outer” regions of the plaque. Note there was a significant loss of synaptophysin immunoreactivity between the outer and middle plaque layers on the contralateral (c) but not inpsilateral, ChABC-injected side (d). For (b) n = 5 mice, for (c) and (d) n = 7 mice, * p < 0.05; ***p < 0.001