Fig. 9

Assessing synaptic densities using the high-resolution array tomography approach. a Representative 3D reconstruction of thirty 70 nm sections from the LBC1936 BA41/42 cortical tissue, stained for synaptophysin, PSD95, AW7 and DAPI. Note the lack of AW7 staining, but strong synaptic labeling. Panels at the bottom show the individual synaptic channels from one of the analysed crops from BA41/42. The white circle highlights a synaptic “snowman”. b Representative 3D reconstruction of twenty-six 70 nm sections from the LBC1936 BA41/42 cortical tissue, stained for synaptophysin, PSD95, AW7 and DAPI. Note the weak synaptic labeling and large AW7-positive amyloid plaque. Large scale bar = 25 μm, small scale bar = 1 μm. c Histogram summarizing the PSD density values from 5 cortical regions from the LBC1936 brain (black bars) and an AD brain (grey bars). d Histogram summarizing synaptophysin densities from the LBC1936 brain and an AD brain. e Histogram summarizing the PSD volume data from 5 cortical regions from the LBC1936 brain and an AD brain. f Histogram summarizing the synaptophysin volume data from the LBC1936 cortex and an AD cortex. Histograms represent the median of all values generated from all individual crops within that region