Fig. 6
Axonal and dendritic transport of BDNF is impaired in APPOSK-expressing tau knockout neurons. Tau knockout mouse primary neurons were doubly transfected with APP-EGFP and BDNF-mRFP. Transport of BDNF in living neurons was recorded for 25 s, and those in 100-μm segments of the axon (a) and 45-μm segments of the dendrite (c) were analyzed. b Compared with mock-transfectants, APPWT-expressing cells showed no differences in BDNF transport in axons. In contrast, APPOSK-expressing cells showed a significant decrease of anterograde, retrograde, and total axonal flux of BDNF. d In dendrites, both APPWT- and APPOSK-expressing cells showed a decrease of BDNF transport, but the differences were significant only in APPOSK-expressing cells