Fig. 2

GCLCsh blocks aberrant growth in TSC in vivo model. a Scheme of in vivo electroporation. b Representative images of cells in rostral migratory stream obtained in in vivo experiments. Newborn rats were electroporated with pSuper vector or TSC2sh#2 together with pSuper or GCLCsh#1. Plasmid encoding GFP was used to visualize modified cells. Scale bar: 50 μm. c Exemplary 3D reconstructions of GFP-positive cells in rostral migratory stream. Scale bar: 3 μm. d Quantification of cell soma volume of cells migrating in the rostral migratory stream. Plot represents mean +/−SEM. ***p < 0.001 in Kruskal-Wallis with Dunn’s post-hoc test. Sample sizes for experimental groups are provided in Supplementary materials and methods (Additional file 1)