Fig. 7

Co-expression of TDP-43^WT and TDP-43^Q331K in mice results increases levels of pathological phosphorylated TDP-43 and C-terminal fragments. a Western blotting from brains demonstrated an increase in phosphorylated TDP-43 (pTDP) in TDP-43^WTxQ331K mice, compared to their single and non-transgenic littermates (asterisks indicate non-specific bands). b Quantification of pTDP showing a significant increase only in the TDP-43^WTxQ331K animals (* p < 0.05 vs. all other genotypes). c Western blotting of total TDP-43 showing an increase in the levels of both 25 kDa and to a lesser extent 35 kDa TDP-43 fragments (arrows) in transgenic mice compared to their non-transgenic littermates (asterisks indicate non-specific bands). d Quantification of the 25 kDa and 35 kDa fragments, demonstrating an expression and mutation dependent increase in both fragments that reaches significance in TDP-43^Q331K and TDP-43^WTxQ331K animals (* p < 0.05 vs. NTg animals; †p < 0.05 vs. TDP-43^WT animals)