Table 2 Summary of microglia neuropathological assessment
CD68 positive pixels (Fig. 2) | |||||||
|---|---|---|---|---|---|---|---|
WM | sub | CA1 | CA2/3 | CA4 | DG | hipp ave | |
NC (N = 9) | 7.5 ± 4 | 2.5 ± 1.6 | 2.6 ± 2.2 | 3.2 ± 2.1 | 2.8 ± 2.2 | 2.4 ± 1.6 | 3.5 ± 2.1 |
HS-aging (N = 7) | 8.7 ± 2.4 | 6 ± 3.5 | 5.4 ± 2.1 | 4.1 ± 2.1 | 3.4 ± 1.2 | 2.4 ± 1.2 | 5 ± 1.4 |
AD (N = 7) | 10.6 ± 3.2 | 6.2 ± 2 | 5.3 ± 1.5 | 4.2 ± 2.6 | 3.5 ± 1.2 | 4.8 ± 2 | 5.8 ± 1 |
AD + HS (N = 4) | 8.7 ± 3.6 | 5.5 ± 2.6 | 5.8 ± 2.1 | 3.2 ± 1.2 | 2.4 ± 1.3 | 2.5 ± 1.4 | 4.7 ± 1.2 |
DLB (N = 12) | 7.6 ± 2.8 | 2.7 ± 1 | 2.3 ± 0.8 | 3.4 ± 2.3 | 2.7 ± 1.1 | 2.2 ± 0.3 | 3.5 ± 0.9 |
CD68 nuclear algorithm (Fig. 4) | |||||||
NC (N = 9) | 54.7 ± 52.1 | 13.9 ± 17.9 | 16 ± 18.5 | 17 ± 18.3 | 20 ± 24 | 10.7 ± 11.3 | 22.1 ± 22.1 |
HS-aging (N = 7) | 54.8 ± 45.1 | 34.4 ± 33.6 | 30.2 ± 20.9 | 20.4 ± 19.8 | 19.2 ± 11.9 | 8.1 ± 7.8 | 27.8 ± 21.2 |
AD (N = 7) | 100.2 ± 68.4 | 45.8 ± 35.8 | 29.2 ± 16.8 | 16.4 ± 7.5 | 17.3 ± 13.3 | 26.3 ± 18.1 | 39.2 ± 22.4 |
AD + HS (N = 4) | 32.4 ± 15.8 | 27.2 ± 22.4 | 28.7 ± 26.6 | 15.4 ± 14.4 | 8 ± 6.8 | 3.8 ± 5.2 | 19.2 ± 13.7 |
DLB (N = 12) | 53.3 ± 35 | 11.8 ± 9 | 11.6 ± 7.7 | 15.6 ± 11.1 | 13.3 ± 8.1 | 8.9 ± 5.9 | 19.1 ± 10.1 |
IBA1 nuclear algorithm (Fig. 5) | |||||||
NC (N = 9) | 101.9 ± 30.2 | 66.5 ± 14.3 | 60.8 ± 22.9 | 74 ± 11.7 | 82.7 ± 16.8 | 80.5 ± 18.7 | 77.7 ± 11.7 |
HS-aging (N = 7) | 143.7 ± 80.8 | 84.7 ± 39.4 | 122.1 ± 63.5 | 142.8 ± 37.3 | 110.9 ± 50.3 | 92.3 ± 39.1 | 116.1 ± 45.4 |
AD (N = 7) | 87.8 ± 55.4 | 84.1 ± 44.2 | 64.2 ± 16.2 | 75.5 ± 19.2 | 43.2 ± 27.2 | 50.5 ± 37.2 | 67.5 ± 26.7 |
AD + HS (N = 4) | 97.9 ± 25.8 | 116.4 ± 74.9 | 108.2 ± 42.1 | 132.4 ± 64.6 | 94.3 ± 47.1 | 98.9 ± 47.1 | 108 ± 41.9 |
DLB (N = 12) | 103.2 ± 55.6 | 69.3 ± 35.6 | 70 ± 32 | 74.2 ± 46.7 | 83.8 ± 48 | 74.5 ± 43.9 | 79.2 ± 40.7 |
IBA1 positive pixels (Fig. 6) | |||||||
NC (N = 9) | 2.8 ± 0.8 | 2.6 ± 0.9 | 2.4 ± 0.7 | 3.1 ± 0.8 | 3.3 ± 1 | 3.1 ± 0.8 | 2.9 ± 0.7 |
HS-aging (N = 7) | 4 ± 1.8 | 2.5 ± 0.9 | 3.2 ± 1.1 | 3.9 ± 0.8 | 3.5 ± 1.3 | 3.1 ± 1.1 | 3.3 ± 1 |
AD (N = 7) | 2.7 ± 1.2 | 2.5 ± 1.1 | 2.2 ± 0.6 | 3.1 ± 1 | 2 ± 1.1 | 2.1 ± 0.8 | 2.4 ± 0.7 |
AD + HS (N = 4) | 3.6 ± 0.8 | 3.4 ± 2.1 | 3.6 ± 1.1 | 3.9 ± 1.2 | 3.1 ± 0.7 | 3.3 ± 1 | 3.5 ± 1 |
DLB (N = 12) | 2.8 ± 0.8 | 1.9 ± 0.6 | 1.9 ± 0.5 | 2.3 ± 0.8 | 2.6 ± 0.9 | 2.5 ± 0.9 | 2.3 ± 0.7 |
Morphological assessment of IBA1+ microglia in CA1 region (Fig. 7) | |||||||
ramified | hypertrophic | dystrophic | rod-shaped | amoeboid | total | ||
NC (N = 9) | 16.9 ± 9.8 | 3.7 ± 7.1 | 4.4 ± 3.9 | 2.1 ± 4.1 | 3.7 ± 5 | 30.8 ± 10.3 | |
HS-aging (N = 7) | 2.6 ± 2.6 | 13.8 ± 11.9 | 32.2 ± 22 | 2.4 ± 5.1 | 8.8 ± 8.8 | 59.9 ± 29.8 | |
AD (N = 7) | 19.7 ± 10.4 | 4.5 ± 6.1 | 11.2 ± 9.7 | 2.6 ± 2.7 | 4.5 ± 3.6 | 42.4 ± 11.5 | |
AD + HS (N = 4) | 9.8 ± 8.3 | 20.2 ± 13.7 | 19.8 ± 11.2 | 2.6 ± 3.2 | 14.6 ± 12.4 | 67 ± 15.8 | |
DLB (N = 12) | 9.3 ± 6.5 | 2.5 ± 3.6 | 13.9 ± 9.3 | 3.9 ± 4 | 1.9 ± 2.6 | 31.3 ± 8.2 | |