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Table 2 Summary of microglia neuropathological assessment

From: Disease-related microglia heterogeneity in the hippocampus of Alzheimer’s disease, dementia with Lewy bodies, and hippocampal sclerosis of aging

CD68 positive pixels (Fig. 2)
  WM sub CA1 CA2/3 CA4 DG hipp ave
NC (N = 9) 7.5 ± 4 2.5 ± 1.6 2.6 ± 2.2 3.2 ± 2.1 2.8 ± 2.2 2.4 ± 1.6 3.5 ± 2.1
HS-aging (N = 7) 8.7 ± 2.4 6 ± 3.5 5.4 ± 2.1 4.1 ± 2.1 3.4 ± 1.2 2.4 ± 1.2 5 ± 1.4
AD (N = 7) 10.6 ± 3.2 6.2 ± 2 5.3 ± 1.5 4.2 ± 2.6 3.5 ± 1.2 4.8 ± 2 5.8 ± 1
AD + HS (N = 4) 8.7 ± 3.6 5.5 ± 2.6 5.8 ± 2.1 3.2 ± 1.2 2.4 ± 1.3 2.5 ± 1.4 4.7 ± 1.2
DLB (N = 12) 7.6 ± 2.8 2.7 ± 1 2.3 ± 0.8 3.4 ± 2.3 2.7 ± 1.1 2.2 ± 0.3 3.5 ± 0.9
CD68 nuclear algorithm (Fig. 4)
NC (N = 9) 54.7 ± 52.1 13.9 ± 17.9 16 ± 18.5 17 ± 18.3 20 ± 24 10.7 ± 11.3 22.1 ± 22.1
HS-aging (N = 7) 54.8 ± 45.1 34.4 ± 33.6 30.2 ± 20.9 20.4 ± 19.8 19.2 ± 11.9 8.1 ± 7.8 27.8 ± 21.2
AD (N = 7) 100.2 ± 68.4 45.8 ± 35.8 29.2 ± 16.8 16.4 ± 7.5 17.3 ± 13.3 26.3 ± 18.1 39.2 ± 22.4
AD + HS (N = 4) 32.4 ± 15.8 27.2 ± 22.4 28.7 ± 26.6 15.4 ± 14.4 8 ± 6.8 3.8 ± 5.2 19.2 ± 13.7
DLB (N = 12) 53.3 ± 35 11.8 ± 9 11.6 ± 7.7 15.6 ± 11.1 13.3 ± 8.1 8.9 ± 5.9 19.1 ± 10.1
IBA1 nuclear algorithm (Fig. 5)
NC (N = 9) 101.9 ± 30.2 66.5 ± 14.3 60.8 ± 22.9 74 ± 11.7 82.7 ± 16.8 80.5 ± 18.7 77.7 ± 11.7
HS-aging (N = 7) 143.7 ± 80.8 84.7 ± 39.4 122.1 ± 63.5 142.8 ± 37.3 110.9 ± 50.3 92.3 ± 39.1 116.1 ± 45.4
AD (N = 7) 87.8 ± 55.4 84.1 ± 44.2 64.2 ± 16.2 75.5 ± 19.2 43.2 ± 27.2 50.5 ± 37.2 67.5 ± 26.7
AD + HS (N = 4) 97.9 ± 25.8 116.4 ± 74.9 108.2 ± 42.1 132.4 ± 64.6 94.3 ± 47.1 98.9 ± 47.1 108 ± 41.9
DLB (N = 12) 103.2 ± 55.6 69.3 ± 35.6 70 ± 32 74.2 ± 46.7 83.8 ± 48 74.5 ± 43.9 79.2 ± 40.7
IBA1 positive pixels (Fig. 6)
NC (N = 9) 2.8 ± 0.8 2.6 ± 0.9 2.4 ± 0.7 3.1 ± 0.8 3.3 ± 1 3.1 ± 0.8 2.9 ± 0.7
HS-aging (N = 7) 4 ± 1.8 2.5 ± 0.9 3.2 ± 1.1 3.9 ± 0.8 3.5 ± 1.3 3.1 ± 1.1 3.3 ± 1
AD (N = 7) 2.7 ± 1.2 2.5 ± 1.1 2.2 ± 0.6 3.1 ± 1 2 ± 1.1 2.1 ± 0.8 2.4 ± 0.7
AD + HS (N = 4) 3.6 ± 0.8 3.4 ± 2.1 3.6 ± 1.1 3.9 ± 1.2 3.1 ± 0.7 3.3 ± 1 3.5 ± 1
DLB (N = 12) 2.8 ± 0.8 1.9 ± 0.6 1.9 ± 0.5 2.3 ± 0.8 2.6 ± 0.9 2.5 ± 0.9 2.3 ± 0.7
Morphological assessment of IBA1+ microglia in CA1 region (Fig. 7)
  ramified hypertrophic dystrophic rod-shaped amoeboid total  
NC (N = 9) 16.9 ± 9.8 3.7 ± 7.1 4.4 ± 3.9 2.1 ± 4.1 3.7 ± 5 30.8 ± 10.3  
HS-aging (N = 7) 2.6 ± 2.6 13.8 ± 11.9 32.2 ± 22 2.4 ± 5.1 8.8 ± 8.8 59.9 ± 29.8  
AD (N = 7) 19.7 ± 10.4 4.5 ± 6.1 11.2 ± 9.7 2.6 ± 2.7 4.5 ± 3.6 42.4 ± 11.5  
AD + HS (N = 4) 9.8 ± 8.3 20.2 ± 13.7 19.8 ± 11.2 2.6 ± 3.2 14.6 ± 12.4 67 ± 15.8  
DLB (N = 12) 9.3 ± 6.5 2.5 ± 3.6 13.9 ± 9.3 3.9 ± 4 1.9 ± 2.6 31.3 ± 8.2  
  1. Values represent mean ± SD for the quantification of CD68 and IBA1 immunohistochemistry. Data is plotted in the indicated figures