Fig. 4
Digital neuropathological quantification using nuclear algorithm of number of large round CD68+ cells in the hippocampus of autopsy cases. Representative example of (a) CD68 staining and a digitally generated mark-up showing the ability of the nuclear algorithm to detect the staining of the large round cells, but not smaller cells or processes. Digital neuropathological quantification of the CD68 staining using the nuclear algorithm to detect the staining is shown for the (b) WM, (c) sub (F4,38 = 2.9934; p = 0.0321), (d) CA1, (e) CA2/3, (f) CA4, (g) DG (F4,38 = 4.3393; p = 0.0061), and for the (h) average of the hippocampal formation. Circles represent an individual case, with mean and SEM shown for the group. Statistical comparisons: *p < 0.05 compared to AD cases. (i) Heatmap summarizes the results shown in (b-h) (also see Table 2)